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Structural basis for the inhibition of NF-B-DNA binding by 4-OXO-DHA Employing our experimental approach 35 , pure p50 protein (Active Motif North America, Carlsbad, CA) at a concentration of 3 nM was incubated with various concentrations of 4-OXO-DHA (6.25, 12.5, 25, 50, 100, 200 M) at ambient temperature for 30 min with 10% DMSO as vehicle without the addition of 4-OXO-DHA in control samples

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